Book Detail:
Author: P. Traub
Publisher: Springer Science & Business Media
ISBN: 3642702309
Size: 11.33 MB
Format: PDF, Kindle
Release Date: 2012-12-06
Category: Science
Language: en
View: 5523
Status: Available

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Book Description
Download PDF Intermediate Filaments eBook. You can read online on your kindle, Android, iPhone, iPad. Research on cytoskeletal elements of eukaryotic cells has been expand ing explosively during the past 5 to 10 years. Due largely to the employment of electron and immunofluorescent microscopy, significant results have been obtained which have provided interesting new insights into the dynamics of nucleated cells at the structural, physiological, as well as developmental levels. While a substantial amount of knowledge has accumulated on the function of microfilaments and microtubules, the roles of the third major class of cytoskeletal structures in vertebrate cells, the intermediate filaments, have largely resisted clarification. The investigation of cultured cells and of tissues from various developmental stages has furnished a host of information on the inter-and intracellular distribution of the different types of intermediate filaments and led to the contention that they have a structural and organizing function in the cytoplasm of vertebrate cells. However, the results of recent experimen that vertebrate cells can function perfectly in the tation have shown complete absence of cytoplasmically extended intermediate filament meshworks. It is legitimate to suppose, therefore, that their function in vertebrate cells is much more subtle and complex than generally presumed. Our interest in the structure and function of intermediate filament proteins was initiated approximately 7 years ago while working on the regulation of macromolecular synthesis in picornavirus-infected mam malian cells. In attempts to demonstrate virus-induced changes in the nuclear protein components of the host cells, the nonionic detergent extraction method was used to purify nuclei.

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Book Detail:
Author: Normand Marceau
Publisher: Biota Publishing
ISBN: 1615045414
Size: 13.33 MB
Format: PDF, ePub, Mobi
Release Date: 2014-06-01
Category: Science
Language: en
View: 347
Status: Available

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Book Description
Download PDF Intermediate Filaments eBook. You can read online on your kindle, Android, iPhone, iPad. Intermediate filaments (IFs), in concert with microfilaments (MFs) and microtubules (MTs), form the cytoskeleton, and each of these fibrillar networks exhibits rather unique structural and functional characteristics. Intermediate filaments were discovered in eukaryotic cells in the late 1960s, and their name comes from the fact that their diameter is intermediate between MFs and MTs. In contrast to the latter, IFs constitute a network that extends from the nuclear envelope throughout the cytoplasm, and in many cases, interact with cell surface domains involved in cell-cell and cell- matrix interactions. Several key features of their expression, assembly, structure and dynamics are highlighted in this eBook. For instance, IF proteins are encoded by several genes, which are classified into six types reflecting the tissues (cells) of origin. Moreover, IF proteins contain a conserved central α-helical (rod) domain flanked by N-terminal (head) and C-terminal (tail) globular domains that enables assembly of fibrous IFs exhibiting a tripartite structure. Although the rod domain is responsible for the formation of the coiled-coil framework and yields the main driving force during the IF protein assembly, the head and tail domains contribute to most of the structural heterogeneity of IF organization and undergo several types of post-translational modifications. Furthermore, the development of gene targeting methods to genetically knockout the expression of the IF genes in mice has uncovered the mechanical versus non-mechanical features of the IF networks, namely, their involvement in cell response to diverse forms of stress, growth stimulation, migration, or death insults. Finally, there is accumulating evidence revealing that the tissue and cell-type expression of IF genes reflects itself in the presence of causal or predisposition mutations responsible for numerous human tissue-specific diseases, known as IF-pathies. Table of Contents: List of Abbreviations / Introduction / IFs as a Multigene Family of Filamentous Proteins / Nuclear Lamina / IF Functional Interplay with Cell Surface Domains and Organelles / IFs and Cell Specialization / IF Relevance to Human Diseases / Conclusion / References / Author Biographies

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Book Detail:
Author: Harald Herrmann
Publisher: Springer Science & Business Media
ISBN: 9780306458545
Size: 76.91 MB
Format: PDF
Release Date: 1998-09-30
Category: Science
Language: en
View: 5451
Status: Available

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Book Description
Download PDF Intermediate Filaments eBook. You can read online on your kindle, Android, iPhone, iPad. Comprises 20 contributions on intermediate filament (IF) proteins, providing a comprehensive overview of the essentials of IF research and very recent developments, including many previously unpublished concepts and results. Representative topics include intermediate filament organization during oogenesis and early development in the clawed frog, lessons from keratin transgenic and knockout mice, cytokeratins as markers of differentiation in the diagnosis of epithelial tumors, vimentin and lipid metabolism, and nuclear lamin- binding proteins. Annotation copyrighted by Book News, Inc., Portland, OR

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Book Detail:
Author:
Publisher: Academic Press
ISBN: 0128034912
Size: 25.26 MB
Format: PDF, Mobi
Release Date: 2016-01-18
Category: Medical
Language: en
View: 4553
Status: Available

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Book Description
Download PDF Intermediate Filament Proteins eBook. You can read online on your kindle, Android, iPhone, iPad. Intermediate Filament Proteins, the latest volume in the Methods in Enzymology series covers all the intermediate filaments in vertebrates and invertebrates, providing a unique understanding of the multiple different tissue-specific intermediate filaments. This volume also covers the latest methods that are currently being used to study intermediate filament protein function and dynamics. It will be an important companion for any experimentalist interesting in studying this protein family in their cell or organism model system. Focuses on intermediate filaments, including the latest information Provides an up-to-date understanding of the field Contains contributions from the major scientists working and publishing in the field

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Book Detail:
Author: M. Bishr Omary
Publisher: Gulf Professional Publishing
ISBN: 9780125641739
Size: 55.40 MB
Format: PDF, ePub
Release Date: 2004-12-02
Category: Science
Language: en
View: 6759
Status: Available

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Book Description
Download PDF Intermediate Filament Cytoskeleton eBook. You can read online on your kindle, Android, iPhone, iPad. Intermediate filaments are a large family of proteins that are the cytoskeletal elements involved in a number of skin, liver, neuromuscular, cardiac, eye and hair diseases. Intermediate filament genes are regulated in a tissue-and cell type-specific manner and their polymerized protein products protects the cells and tissue they are part of against a variety of mechanical and nonmechanical stresses. This book provides a comprehensive resource of methodology essentials, describing a variety of essential tools and assays for studying intermediate filaments. The book provides user-friendly advice and protocols covering all aspects of intermediate filaments including protein isolation and structure, protein and gene regulation, relationship to disease and apoptosis, and associated proteins. Both mammalian and non-mammalian systems and animal models are covered, making this book a must-have for any investigator wishing to study IF genes or their protein products. * Covers intermediate filaments from crystallography, protein chemistry, cell and molecular biology, microrheology, gene regulation, to animal models and human disease * Practical and user-friendly with detailed "how-to-protocols and "tricks of the trade" * Includes detailed tables of useful reagents, vendors and web links

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Book Detail:
Author: Jesus Paramio
Publisher: Springer Science & Business Media
ISBN: 0387337814
Size: 36.88 MB
Format: PDF, Mobi
Release Date: 2007-06-07
Category: Science
Language: en
View: 5358
Status: Available

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Book Description
Download PDF Intermediate Filaments eBook. You can read online on your kindle, Android, iPhone, iPad. Intermediate Filaments focuses on desmin and other intermediate filaments in disease and health. This new volume will serve as a resource for graduate students and researchers in the field.

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Book Detail:
Author: R.D. Goldman
Publisher: Springer Science & Business Media
ISBN: 1475796048
Size: 13.57 MB
Format: PDF, ePub, Docs
Release Date: 2013-06-29
Category: Science
Language: en
View: 6585
Status: Available

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Book Description
Download PDF Cellular And Molecular Biology Of Intermediate Filaments eBook. You can read online on your kindle, Android, iPhone, iPad. Research activity on intermediate filaments (IF) has increased dramatically over the past decade. For the most part, this surge of interest is due to their identification as ubiquitous constituents of the cytoskeleton and karyoskeleton (nuclear matrix) of eukaryotic cells and the fact that we know very little regarding their functions. In sharp contrast to the other major cytoskeletal systems, microfilaments and microtubules, IF exhibit a high degree of heterogeneity with regard to their protein subunit composition. Indeed, one can only marvel at the number of different IF polypeptides, their associated proteins (IFAP) and, consequently, the number of genes involved in encoding the multiple constituents of the various IF networks found in different cell types. The chapters in this book demonstrate how various experimental approaches involv ing cellular, molecular, biochemical, and immunological methods have been utilized to generate information regarding the structure and function of IF. To this end, we have gathered together chapters from experts in the major fields of IF research. In each chapter, the authors have combined reviews of the available scientific literature with their own ideas on current and future directions for IF research. The chapters have been divided into five major sections which are concerned with the subcellular organization of IF, the molecular structure of IF, the differential expression of IF genes, descriptions of associ ated proteins involved in the intracellular organization of IF, and finally an analysis of the changes seen in IF in pathological conditions.

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Book Detail:
Author:
Publisher: Academic Press
ISBN: 0128034904
Size: 64.98 MB
Format: PDF
Release Date: 2016-01-14
Category: Medical
Language: en
View: 3469
Status: Available

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Book Description
Download PDF Intermediate Filament Associated Proteins eBook. You can read online on your kindle, Android, iPhone, iPad. Intermediate Filament Associated Proteins, the latest volume in the Methods in Enzymology series, continues the legacy of this premier serial with quality chapters authored by leaders in the field. This volume covers research methods in intermediate filament associated proteins and contains sections on such topics as lamin-associated proteins, intermediate filament-associated proteins and plakin, and other cytoskeletal cross-linkers. Continues the legacy of this premier serial with quality chapters authored by leaders in the field Covers research methods in intermediate filament associated proteins and contains sections on such topics as lamin-associated proteins, intermediate filament-associated proteins and plakin, and other cytoskeletal cross-linkers

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Book Detail:
Author: Yaming Jiu
Publisher: Frontiers Media SA
ISBN: 2832510469
Size: 37.59 MB
Format: PDF, Kindle
Release Date: 2023-01-02
Category: Science
Language: en
View: 4910
Status: Available

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Book Description
Download PDF Intermediate Filaments Structure Function And Clinical Significance eBook. You can read online on your kindle, Android, iPhone, iPad.

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Book Detail:
Author: Heide Schatten
Publisher: Springer
ISBN: 1493929046
Size: 77.32 MB
Format: PDF, ePub, Mobi
Release Date: 2015-08-21
Category: Science
Language: en
View: 1236
Status: Available

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Book Description
Download PDF The Cytoskeleton In Health And Disease eBook. You can read online on your kindle, Android, iPhone, iPad. This volume addresses the structural and functional roles of the cytoskeleton and its dysfunctions which often lead to disease. It provides thorough discussion of microtubules, microfilaments, intermediate filaments, and cytoskeletal functions and dysfunctions in different organ systems. Comprehensive yet concise. The Cytoskeleton In Health And Disease presents cutting-edge discoveries balanced with background information and highlights the new aspects of the research and its impact on the design of new strategies or the identification of new targets for therapeutic intervention. There is a significant need for a book on this topic, as interest in the cytoskeleton continues to grow as causes and cures for cytoskeletal diseases are further explored in biomedical research. This book is essential reading for scientists, students, and teachers interested in expanding their knowledge related to the cytoskeleton. New researchers entering the field will find classic and well as contemporary information not easily found in the current literature or internet resources.

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Book Detail:
Author:
Publisher: ScholarlyEditions
ISBN: 146494072X
Size: 48.68 MB
Format: PDF
Release Date: 2012-01-09
Category: Medical
Language: en
View: 2340
Status: Available

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Book Description
Download PDF Intermediate Filament Proteins Advances In Research And Application 2011 Edition eBook. You can read online on your kindle, Android, iPhone, iPad. Intermediate Filament Proteins: Advances in Research and Application: 2011 Edition is a ScholarlyBrief™ that delivers timely, authoritative, comprehensive, and specialized information about Intermediate Filament Proteins in a concise format. The editors have built Intermediate Filament Proteins: Advances in Research and Application: 2011 Edition on the vast information databases of ScholarlyNews.™ You can expect the information about Intermediate Filament Proteins in this eBook to be deeper than what you can access anywhere else, as well as consistently reliable, authoritative, informed, and relevant. The content of Intermediate Filament Proteins: Advances in Research and Application: 2011 Edition has been produced by the world’s leading scientists, engineers, analysts, research institutions, and companies. All of the content is from peer-reviewed sources, and all of it is written, assembled, and edited by the editors at ScholarlyEditions™ and available exclusively from us. You now have a source you can cite with authority, confidence, and credibility. More information is available at http://www.ScholarlyEditions.com/.

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Book Detail:
Author: A.D. Bershadsky
Publisher: Springer Science & Business Media
ISBN: 1468452789
Size: 36.72 MB
Format: PDF, Mobi
Release Date: 2012-12-06
Category: Science
Language: en
View: 1436
Status: Available

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Book Description
Download PDF Cytoskeleton eBook. You can read online on your kindle, Android, iPhone, iPad. This book, like other monographs of the Cellular Organelles series, is not a comprehensive review, but an introduction to the study of cytoskeleton. Accordingly, we describe only the main facts and concepts related to cyto skeleton. Needless to say, selection and interpretation was influenced by the personal interests and opinions of the authors, although we attempted to be as fair as possible. We wished to familiarize the reader not only with well established facts, but with current unsolved problems. Therefore, the words "possibly," "maybe," "not known," and "not clear" are much more frequent in this text than in many others. In accordance with the style of the series, relatively short lists of addi tional readings are given at the end of each chapter; these lists contain mostly the recent reviews and a few original papers describing certain phenomena in detail. Few references are cited in the text; these citations are given to help the reader find the source of certain new data and theories, which are not discussed at length in the reviews. In contrast, many well-established facts and widely known theories are not cited.

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Book Detail:
Author:
Publisher:
ISBN:
Size: 78.89 MB
Format: PDF, Mobi
Release Date: 2014
Category:
Language: en
View: 2991
Status: Available

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Book Description
Download PDF Mechanics Of Intermediate Filaments eBook. You can read online on your kindle, Android, iPhone, iPad. The eukariotic cytoskeleton consists of three major filament types: actin filaments, microtubules and intermediate filaments. While the first two filament types are highly conserved, intermediate filaments vary between cell types. Here, we focus on the intermediate filament vimentin, which occurs mesenchymal cells. A defining feature of intermediate filaments is their assembly process. The filaments assemble in a hierarchical manner from tetrameric complexes of half-staggered antiparalell coiled-coils. These tetrameric structures then align laterally to form unit-length filaments which in t...

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Book Detail:
Author: Gulsen Colakoglu
Publisher:
ISBN:
Size: 60.67 MB
Format: PDF, Kindle
Release Date: 2009
Category: Cytoplasmic filaments
Language: en
View: 5287
Status: Available

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Book Description
Download PDF Assembly Dynamics Of Intermediate Filaments eBook. You can read online on your kindle, Android, iPhone, iPad. Abstract: The cytoskeleton of eukaryotic cells comprises three classes of protein polymers: actin filaments, microtubules and intermediate filaments. Actin filaments and microtubules are known to lengthen and shorten by addition and loss of subunits at their ends. In contrast, several studies suggest that intermediate filaments may lengthen by end-to-end annealing of pre-existing filaments and that addition and loss of subunits is not confined to the filament ends. To address these hypotheses, we expressed mCherry-tagged, GFP-tagged or photoactivatable GFP-tagged neurofilament or vimentin proteins in cultured SW13 vim- cells, which lack endogenous cytoplasmic intermediate filaments. To test for annealing, we used polyethylene glycol-induced cell fusion to create cells containing a mixture of red and green filaments. Within five hours, we observed many examples of single intermediate filaments comprised of alternating red and green segments, indicating that end-to-end annealing is a common event. To determine the site of subunit incorporation, we used cell fusion to create cells containing a mixture of red and photoactivatable green fluorescent filaments and then used whole-cell photoactivation to mark those filaments that lacked red fluorescence. Alternatively, we co-expressed red and photoactivatable green fluorescent neurofilament proteins in the same cells to make filaments containing both red and green fluorescence, then photobleached the red fluorescence and used partial-cell photoactivation to mark a sub-population of the bleached filaments. In both cases, we observed the incorporation of newly synthesized or pre-existing red fluorescent intermediate filament subunits along the length of the pre-existing photoactivated green fluorescent filaments over a period of eight to twenty hours, and no preferential addition of subunits to the filament ends. We confirmed our results in SW13 vim+ cells with endogenous vimentin networks and in MFT-16 fibroblasts derived from vimentin knockout mice. These data provide the first direct evidence that intermediate filaments in cells can elongate by end-to-end annealing and that filaments can incorporate subunits along their length, a process that we term intercalary subunit exchange.

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Book Detail:
Author: Johanna Lena Block
Publisher: Göttingen University Press
ISBN: 3863953738
Size: 12.39 MB
Format: PDF, ePub
Release Date: 2018
Category:
Language: en
View: 5184
Status: Available

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Book Description
Download PDF Stress Strain Behavior Of Single Vimentin Intermediate Filament eBook. You can read online on your kindle, Android, iPhone, iPad. Cells are the basic unit of living organisms and consist of a cytoplasm, which is enclosed by a membrane. As building blocks of life with a plethora of functions, cells have to be equipped with a high degree of mechanical resistance, durability, and variability. In eukaryotic cells three filamentous protein types – actin filaments, microtubules, and intermediate filaments (IFs) – form the so-called cytoskeleton, a network that is known to play a key role for the mechanical properties of cells. Among the three filament systems, IFs are special in terms of, for example, their hierarchical architecture, and their cell-type specific expression. In this thesis, vimentin, an IF mostly found in mesenchymal cells, is studied as a model system to learn more about the mechanical properties of IFs, and the underlying mechanisms that determine their robustness, stiffness, and flexibility. Using a combination of optical trapping and atomic force microcopy experiments and stochastic and numerical modelling, vimentin is found to possess impressive physical properties, such as an extendibility of about 3.6 times its initial length and a tensile memory that can be directly linked to the molecular architecture of the protein and the hierarchical construction of the filament. The experimental results show a clear loading-rate- and strain-dependent behavior of single vimentin IFs supporting the hypothesis that vimentin acts as a “safety belt” for cells, protecting them especially at large and fast deformations. The potential to dissipate a large amount of energy that is attributed to distinct non-equilibrium unfolding and refolding of the α-helices, which are the main structural feature of the vimentin monomer, enables vimentin to act as a shock absorber when exposed to large deformations. In case of cyclic deformations, such as in the cardiovascular system, the observed tensile memory could potentially help cells to be compliant with the repeated strain. In conclusion, vimentin is found to display highly interesting and diverse mechanical properties depending on the applied stress that could be linked to the molecular architecture of the filaments and enable vimentin to determine the mechanical properties of cells to a large extend.

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Book Detail:
Author:
Publisher: Elsevier
ISBN: 9780080469393
Size: 58.35 MB
Format: PDF, Kindle
Release Date: 2011-09-02
Category: Science
Language: en
View: 5996
Status: Available

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Book Description
Download PDF Aspects Of The Cytoskeleton eBook. You can read online on your kindle, Android, iPhone, iPad. This volume provides an overview of the cytoskeleton particularly on the fundamental role the cytoskeleton plays in the regulation of cell structure and function. This book represents new trends in cytoskeletal research that go beyond the traditional approach of identifying new proteins in the cytoskeleton, but actually define how these proteins interact with signaling pathways. While the major emphasis in this volume remains on the microfilament structure, some discussion has been included in this volume to illustrate the similarities and differences between the three cytoskeletal elements namely the actin microfilament, the intermediate filaments and the microtubules.

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Book Detail:
Author: Anna Veronika Schepers
Publisher: Universitätsverlag Göttingen
ISBN: 3863955676
Size: 43.57 MB
Format: PDF, Kindle
Release Date: 2022
Category:
Language: en
View: 2679
Status: Available

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Book Description
Download PDF Intermediate Filament Mechanics Across Scales From Single Filaments To Single Interactions And Networks In Cells eBook. You can read online on your kindle, Android, iPhone, iPad. The mechanical properties of cells are largely determined by the cytoskeleton. The cytoskeleton is an intricate and complex structure formed by protein filaments, motor proteins, and crosslinkers. The three main types of protein filaments are microtubules, actin filaments, and intermediate filaments ( IFs ). Whereas the proteins that form microtubules and actin filaments are exceptionally conserved throughout cell types and organisms, the family of IFs is diverse. For example, the IF protein vimentin is expressed in relatively motile fibroblasts, and keratin IFs are found in epithelial cells. This variety of IF proteins might therefore be linked to the various mechanical properties of different cell types. In the scope of this thesis, I combine studies of IF mechanics on different time scales and in systems of increasing complexity, from single filaments to networks in cells. This multiscale approach allows for the simplification necessary to interpret observations while adding increasing physiological context in subsequent experiments. We especially focus on the tunability of the IF mechanics by environmental cues in these increasingly complex systems. In a series of experiments, including single filament elongation studies, single filament stretching measurements with optical tweezers, filament-filament interaction measurements with four optical tweezers, microrheology, and isotropic cell stretching, we characterize how electrostatic (pH and ion concentration) and hydrophobic interactions (detergent) provide various mechanisms by which the mechanics of the IF cytoskeleton can be tuned. These studies reveal how small changes, such as charge shifts, influence IF mechanics on multiple scales. In combination with simulations, we determine the mechanisms by which charge shifts alter single vimentin filament mechanics and we extract energy landscapes for interactions between single filaments. Such insights will provide a deeper understanding of the mechanisms by which cells can maintain their integrity and adapt to the mechanical requirements set by their environment.

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Book Detail:
Author: Ariane Zamoner
Publisher:
ISBN:
Size: 51.25 MB
Format: PDF, Kindle
Release Date: 2017
Category: Science
Language: en
View: 4096
Status: Available

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Book Description
Download PDF Intermediate Filaments As A Target Of Signaling Mechanisms In Neurotoxicity eBook. You can read online on your kindle, Android, iPhone, iPad. In this chapter, we deal with the current knowledge and important results on the cytoskeletal proteins and their differential regulation by kinases/phosphatases and Ca2+-mediated mechanisms in developmental rat brain. We focus on the misregulation of the phosphorylating system associated with intermediate filament proteins of neural cells and its relevance to cell and tissue dysfunction. Taking into account our findings, we propose that intermediate-filament proteins are dynamic structures whose regulation is crucial for proper neural cell function. Given their relevance, they must be regulated in response to extracellular and intracellular signals. The complexity and connection between signaling pathways regulating intermediate-filament dynamics remain obscure. In this chapter, we get light into some kinase/phosphatase cascades downstream of membrane receptors disrupting the dynamics of intermediate filaments and its association with neural dysfunction. However, intermediate filaments do not act individually into the neural cells. Our results evidence the importance of misregulated cytoskeletal crosstalk in disrupting cytoskeletal dynamics and cell morphology underlying neural dysfunction in experimental conditions mimicking metabolic diseases and nongenomic actions of thyroid hormones and as an end point in the neurotoxicity of organic tellurium.

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Book Detail:
Author: Thomas Dean Pollard
Publisher:
ISBN: 9781621820161
Size: 40.30 MB
Format: PDF, ePub
Release Date: 2017
Category: Cell membranes
Language: en
View: 5473
Status: Available

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Book Description
Download PDF The Cytoskeleton eBook. You can read online on your kindle, Android, iPhone, iPad. The cytoskeleton is the intracellular filament system that controls the morphology of a cell, allows it to move, and provides trafficking routes for intracellular transport. It comprises three major filament systems-actin, microtubules, and intermediate filaments-along with a host of adaptors, regulators, molecular motors, and additional structural proteins. This textbook presents a comprehensive and up-to-date view of the cytoskeleton, cataloguing its many different components and explaining how they are functionally integrated in different cellular processes. It starts by laying out the basic molecular hardware, before describing in detail how these components are assembled in cells and linked to neighboring cells and the extracellular matrix to maintain tissue architecture. It then surveys the roles of the cytoskeleton in processes such as intracellular transport, cell motility, signal transduction, and cell division. The book is thus essential reading for students learning about intracellular structure. It also represents a vital reference for all cell and developmental biologists working in this field.

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Book Detail:
Author: Bruce Alberts
Publisher:
ISBN: 9780815332183
Size: 29.95 MB
Format: PDF, ePub, Docs
Release Date: 2004
Category: Cells
Language: en
View: 3598
Status: Available

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Book Description
Download PDF Molecular Biology Of The Cell eBook. You can read online on your kindle, Android, iPhone, iPad.

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